8 thoughts on “P9 – Skariah

  1. This was a really great presentation, you were very well spoken! What do you plan on doing differently in the future to isolate your phage from the corndog phage?

    1. Thank you! In the future, knowing the location from which the soil sample was taken containing the Myoviridae, Rickey, I could take another sample and perform enrichment and DNA isolation again. Unfortunately, there is no way of guaranteeing the success of my second round of DNA isolation without submitting the sample for electron micrography to verify.

    1. Unfortunately, I cannot answer that with certainty. It is likely it is a naturally found phage with very similar morphology to lytic myoviridae, found in the same location the soil sample was taken from.

    1. The goal of phage therapy is to kill antibiotic resistant bacteria using phages. Lytic phages kill their host bacteria.

    1. Thank you! When picking the plaque for DNA isolation, it is likely that we picked both a plaque formed by the myoviridae Rickey and the corndog contaminant phage, since their plaque morphologies are very similar. This likely led to the DNA isolation of the two different kinds of phages.

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