9 thoughts on “P17 – Talbot

  1. What were you expecting to see from your restriction digest? And what would it have told you about the phage?
    Great presentation!

    1. Hi Caleb, we were expecting to see more clearly cut bands in each column to show how many fragments they were each cut into which we could have then clearly counted. This would let us know what cluster and more specifically what sub-cluster our phage would have belonged to.

    1. Hi Talya, our findings tell us what sub-cluster the phage we isolated belongs to. This is important for the field of bacteriophages as well as phage therapy because now we can categorize our phage and file it into the phage database. If we send in a sample to the University of Pittsburgh, doctors and scientists are then able to choose the specific phages they need for phage therapy based on the different sub-clusters.

    1. Hi Will, a temperate phage would have looked cloudy on the plate. It would have a lighter bullseye in the middle and a darker halo around that bullseye.

    1. I just saw someone else wrote the same question so here is another one… How were you and your lab partner able to determine the tail length of the phage? And what did you use to determine the diameter of your phage on the plaque?

      1. Hi Geneva, we were able to determine the tail length after getting our Electron Microscopy photos back. These photos (fig. 4) allow us to see our phage more clearly and actually make it possible to measure the tail length as well as the head diameter. When we measure our chosen phage plaque, we used a small scale ruler in order to measure in millimeters.

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