Hi, temperate phages are actually considered good vectors for gene transmission since they can integrate their genome into the host genome and you can then have lateral gene transfer. This would definitely be worth exploring in the future.
Hi, the techniques we used cut up DNA at recognized sequences and while this can be really helpful in starting the process of narrowing down what cluster the phage is in, these results can be similar for phages of various clusters and mainly works to help narrow down the top three or so clusters. By using the PCR primers it can narrow down those three or so clusters to one.
Hey Pauline! What is the significance of the titer identified in Figure 2? What do the units represent and how does they compare to a standard or point of reference? Are they higher, lower? Thanks!
Hi, the titer tells us how many phage particles would be represented in a microliter of phage lysate. PFU stands for the phage particles and uL stands for microliters. I believe this is a bit higher of a high titer than average.
Great job!
Do you think you would be able to immediately identify your phage as a siphovirdae phage without the exact measurements? Is the head and tail size purely the only way to identify the differences in type of phage?
If it is better to use lytic phages for phage therapy, then what is the use of a temperate phage and would that be worth exploring in the future?
Hi, temperate phages are actually considered good vectors for gene transmission since they can integrate their genome into the host genome and you can then have lateral gene transfer. This would definitely be worth exploring in the future.
How would techniques such as PCR confirm phage clusters better than techniques used in your experiments?
Hi, the techniques we used cut up DNA at recognized sequences and while this can be really helpful in starting the process of narrowing down what cluster the phage is in, these results can be similar for phages of various clusters and mainly works to help narrow down the top three or so clusters. By using the PCR primers it can narrow down those three or so clusters to one.
Hey Pauline! What is the significance of the titer identified in Figure 2? What do the units represent and how does they compare to a standard or point of reference? Are they higher, lower? Thanks!
Hi, the titer tells us how many phage particles would be represented in a microliter of phage lysate. PFU stands for the phage particles and uL stands for microliters. I believe this is a bit higher of a high titer than average.
Great job!
Do you think you would be able to immediately identify your phage as a siphovirdae phage without the exact measurements? Is the head and tail size purely the only way to identify the differences in type of phage?