If we had isolated our bacteriophage, we would have conducted a series of serial dilutions to calculate the titer of the phage, followed by archiving and DNA Isolation.
the main obstacle was just getting plaques to appear on our purification plate, the plates showed no signs of contamination which meant that sterile technique was not the issue, just the phages behavior proved to be extremely difficult to work with.
What are the possible treatments that can be administered once a bacteriophage is successfully isolated?
If we had isolated our bacteriophage, we would have conducted a series of serial dilutions to calculate the titer of the phage, followed by archiving and DNA Isolation.
What were the results that would have been ideal for your phage to have “worked”?
If we had successful results from our Plaque streak purification, we would have had a lawn of plaques on our plates that contained our specific phage.
What was the main obstacle in cultivation of those bacteriophages? Why satisfying results were so difficult to obtain satisfying resoults?
the main obstacle was just getting plaques to appear on our purification plate, the plates showed no signs of contamination which meant that sterile technique was not the issue, just the phages behavior proved to be extremely difficult to work with.