Great question! Phages are very abundant in soil because of all the bacteria found in soil, so this created a high probability of discovering and isolating a phage.
When isolating our phage from the soil we used a process called enrichment, direct isolation is another process that can be used however, we went with enrichment.
When discovering our phage we used a process called enrichment. Another experiment used to isolate would be direct isolation but we went with the enrichment isolation.
Good question, when exposing our temperate phage to stressful conditions such UV light or radiation this can enter our lysogenic (temperate) phage into the lytic life cycle to be used for phage therapy.
A lytic phages more suitable when it comes to phage therapy, the tail fibers of the phage and the proteins on the bacteria cell surface must match to allow for the phage to infect.
What is “morphology” and why does it determine which life cycle the phage goes into? Is there any possibility that morphology alone doesn’t determine this?
Plaque morphology is is actually determined by the life cycle rather than the other way around, plaque morphology refers to the visual characteristics such as size and cloudy/clear plaques.
Why did you use soil to find bacteriophages, is it because of the specific phage you are looking for or because it’s easily accessible?
Great question! Phages are very abundant in soil because of all the bacteria found in soil, so this created a high probability of discovering and isolating a phage.
What process did you use to isolate the bacteriophages from the soil?
When isolating our phage from the soil we used a process called enrichment, direct isolation is another process that can be used however, we went with enrichment.
When discovering our phage we used a process called enrichment. Another experiment used to isolate would be direct isolation but we went with the enrichment isolation.
Why would you research your bacteria with UV?
Good question, when exposing our temperate phage to stressful conditions such UV light or radiation this can enter our lysogenic (temperate) phage into the lytic life cycle to be used for phage therapy.
What makes a phage eligible to be used in phage therapy ?
A lytic phages more suitable when it comes to phage therapy, the tail fibers of the phage and the proteins on the bacteria cell surface must match to allow for the phage to infect.
What is “morphology” and why does it determine which life cycle the phage goes into? Is there any possibility that morphology alone doesn’t determine this?
Plaque morphology is is actually determined by the life cycle rather than the other way around, plaque morphology refers to the visual characteristics such as size and cloudy/clear plaques.