A restriction digest would be a great start to identify the cluster that our phage belongs to! From there, PCR and DNA sequencing would help to confirm this cluster classification
What would a gel that helped to confirm your data look like? What would you be looking for on the gel to determine if your data were good or bad/what class and subclass your phage belongs to?
We would look at a restriction digest gel and expect to see solid bars, and different placements for each lane based on the primers used. As we don’t currently know the cluster of the phage, we can’t predict which primers will cut the DNA, but we do know that phages ultimately placed within the same cluster often have very similar restriction digest gels
We hope that we will be able to isolate the phage lysate from this. We will isolate the DNA and a restriction digest gel which will tell us if the DNA is pure, and also likewise if the phage lysate is good
Do the sizes of the plaques show you how infectious the phage is or does it allow you to determine what types of bacteria could be infected by the phage?
The sizes of the plaques show the “burst size” of the phage, along with its efficiency in infecting bacteria. The type of bacteria which is infected by the phage aren’t necessarily described by the plaque size.
What sorts of further experimentation could help better identify the cluster/subcluster to which MontyDog belongs?
A restriction digest would be a great start to identify the cluster that our phage belongs to! From there, PCR and DNA sequencing would help to confirm this cluster classification
What would a gel that helped to confirm your data look like? What would you be looking for on the gel to determine if your data were good or bad/what class and subclass your phage belongs to?
We would look at a restriction digest gel and expect to see solid bars, and different placements for each lane based on the primers used. As we don’t currently know the cluster of the phage, we can’t predict which primers will cut the DNA, but we do know that phages ultimately placed within the same cluster often have very similar restriction digest gels
What do you hope will happen with the web pattern plate? what results do you expect?
We hope that we will be able to isolate the phage lysate from this. We will isolate the DNA and a restriction digest gel which will tell us if the DNA is pure, and also likewise if the phage lysate is good
Do the sizes of the plaques show you how infectious the phage is or does it allow you to determine what types of bacteria could be infected by the phage?
The sizes of the plaques show the “burst size” of the phage, along with its efficiency in infecting bacteria. The type of bacteria which is infected by the phage aren’t necessarily described by the plaque size.