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7 thoughts on “P33 – Chaplick”
Your presentation was very clear!
Do you know why a restriction digest would help us determine which class of phage this is? How would that work and what would the results tell us?
The restriction digest will involve cutting the DNA with different restriction enzymes. Phage genomes similar to that of the phage we isolated will have the same number of restriction enzyme cuts located in the same (or similar) location. This will help classify what cluster our phage belongs to.
Great presentation! Are there areas or climates that temperate phages are known to be in? Can temperatures affect it or is it just luck of finding one?
To my knowledge, both lytic and temperate phages can be found in any place where bacteria exists, and the bacteriophages can be found while in either the lysogenic or lytic cycle. Environmental stressors such as exposure to toxins or chemicals may cause the phage to excise and enter the lytic cycle.
Overall super great job! I was wondering about the specific distinctions between temperate and lytic phages, and why isolating a temperature phage my have different implications for future research than a lytic phage?
Lytic phages tend to be better for phage therapy purposes, as they always kill the bacteria that they infect. Temperate phages can lyse their host cells and enter the lytic cycle, but this is not always as effective as simply using a cell already prepped for the lytic cycle.
Lytic phages tend to be better for phage therapy, as they kill the bacteria they infect when they lyse the cell. Temperate phages can enter the lytic cycle, but this is not always as effective as using a phage already prepped to undergo the lytic cycle.