6 thoughts on “P33 – Stoughton

  1. Good presentation. What did it take to do the assays you performed and how did you isolate your phage?

    1. We isolated the phage by adding m. smeg bacteria and growth medium to our soil sample, then taking the liquid and plating on a top agar plate.

    1. The most likely answer is cluster A since it is the largest cluster of siphoviridae, but there is always the chance that its something else

  2. Why would the additional tests needed be necessary for identifying your phage? And how would this identification be important for the use of the phage for medical purposes?

    1. More tests are needed because DNA analysis is needed to accurately and definitively identify a phage. By classifying a phage, it tells us what types of bacteria it is likely to infect and what proteins it codes for, which has implications in phage therapy for antibiotic resistant infections or for the potential discovery of new proteins that could further our understanding of molecular biology.

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