Hi Tiffany! Great presentation. Since your phage is temperate, is there any use in further sequencing/experimentation? Also, which cluster do you think your phage is in?
Hello! thanks for your comment. there would be use in further experimentation! where I’m at now, i haven’t been able to see any information about its DNA or genome, so after completing restriction digest or PCR experiments, i will be able to learn more about it’s cluster. additionally, researchers are considering temperate phages for phage therapy so it can still be useful. since I haven’t performed any experiments regarding its DNA, i don’t have any inklings towards what cluster it could be in yet. thanks again!
Hello! thanks for commenting. for lytic phages, the plaques are clear and have a distinct border, with no halo around them, due to the phages lysing the bacteria after infection.
Hello! thanks for commenting. for lytic phages, the plaques will be clear and have distinct borders, with no halo around them, due to lytic phages lysing the bacteria after infection. thanks again!
Hi Tiffany! Great presentation. Since your phage is temperate, is there any use in further sequencing/experimentation? Also, which cluster do you think your phage is in?
Hello! thanks for your comment. there would be use in further experimentation! where I’m at now, i haven’t been able to see any information about its DNA or genome, so after completing restriction digest or PCR experiments, i will be able to learn more about it’s cluster. additionally, researchers are considering temperate phages for phage therapy so it can still be useful. since I haven’t performed any experiments regarding its DNA, i don’t have any inklings towards what cluster it could be in yet. thanks again!
What would you look for appearance wise if you were trying to find bacteriophage in the lytic phase?
Hello! thanks for commenting. for lytic phages, the plaques are clear and have a distinct border, with no halo around them, due to the phages lysing the bacteria after infection.
Hello! thanks for commenting. for lytic phages, the plaques will be clear and have distinct borders, with no halo around them, due to lytic phages lysing the bacteria after infection. thanks again!