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9 thoughts on “P37 – Gupta”
Did you results support your original hypothesis/expectation? In other terms, did you expect the phage to be temperate when isolated?
When we first isolated the phage, we thought we did so from a small, lytic plaque. We thought that our phage was lytic until our first titer assay when some larger plaques formed and it was clear it was temperate.
Are temperate phage more rare than lytic in your experience from this semester? Last semester most of us found lytic phage. Great presentation and congratulations on the discovery!
In my lab session it appeared to be more common to get temperate phages this time around. I think 6 or 7 out of the 9 had temperate.
What is a restriction digest test?
A restriction digest is a test where you cut several samples of phage DNA with different restriction enzymes and running the samples through gel electrophoresis. The number of cuts done by each enzyme can be used to determine the phage’s cluster.
How do you plan to further your experiment in the future in order to better distinguish the phage?
There is a wide array of tests we can do to further categorize the phage. Restriction digests and PCR cluster tests can both be done to determine phage clusters. Phage research can go much further beyond that, as the entire phage genome can be sequenced and annotated if we had a long enough time to work on this project.
Good job. Will you analyze its genome to find out it is in which group of phages ?