Thank you for sharing your research! I just have a few questions: What steps did you take to isolate your phage and get the EM pictures? What are some benefits of your phage being temperate?
The benefit of a temperate phage is the lysogenic cycle, during this cycle instead of destroying the phage outright like the lytic cycle, the phage will infect the hosts DNA directly, so the host cell will slowly change the DNA of the cell so that phage can be produced by the cell until an error occurs, and the cell dies.
Yes, most of the issues came up in our early procedures, and example of this was when we were trying to collect the DNA of our Phage, we need to collect at least 3 mL of our phage to archive but the first time we only got 2 mL and then we got none on the next run through. The third time we tried we collected 6 mL.
Great presentation! Do you have any information on which cluster your phage might belong to?
Unfortunately, I do not due to us getting a bad restriction digest which didn’t allow us to even hypothesis what cluster our phage is a part of.
Thank you for sharing your research! I just have a few questions: What steps did you take to isolate your phage and get the EM pictures? What are some benefits of your phage being temperate?
The benefit of a temperate phage is the lysogenic cycle, during this cycle instead of destroying the phage outright like the lytic cycle, the phage will infect the hosts DNA directly, so the host cell will slowly change the DNA of the cell so that phage can be produced by the cell until an error occurs, and the cell dies.
Do the other properties that the electron microscopy can show also play a role in identifying if its a Siphoviridae phage?
Great presentation! Are there specific guidlines for naming and archiving phages?
Yes, there are a bunch of different rules and regulations that must be followed when naming, the guidelines are here https://phagesdb.org/namerules/
Yes, there a bunch of rules and regulations that need to be followed when naming, they can be found here https://phagesdb.org/namerules/
Were there any issues that you may have faced while doing your experiment?
Yes, most of the issues came up in our early procedures, and example of this was when we were trying to collect the DNA of our Phage, we need to collect at least 3 mL of our phage to archive but the first time we only got 2 mL and then we got none on the next run through. The third time we tried we collected 6 mL.
Great presentation! how does a PCR test help to identify what group a phage belongs to?