Hey Michelle, could you further explain what the significance of the plaque sizes were and how you determined whether or not it was significant research?
The purification experiment is where we scraped a single plaque from the enrichment plate and incubated it in phage buffer to enrich that single plaque. We did this twice in order to make sure we had a single phage specie. The purification experiment may have failed because the plaque we used was a relatively big plaque, so it may have had two species of phage.
We didn’t really have any controls since the experiments we conducted were mostly characterization. We didn’t expect for the purification experiment to have failed actually. But, we did hypothesize that the phage was highly infectious in mycobacterium because we always ended up in with cleared plate.
It’s important that we recognize plaque size because each different phage species, when infecting bacterium, will result in a different plaque size. That is how we were able to deduce that we had two different phages because there were two different sized plaques.
What exactly was the purification experiment and do you have any ideas why it might have failed?
Hi Michelle! Good to see you. Super well done presentation! Was the DNA isolated from a single bacteriophage or a mixture of both?
Hi Michelle, great presentation. Were you able to tell you had 2 different phages because of the size or pattern of the plaques? Thanks!
Hi Michelle, what were some of your controls in this experiment, just curious. And did you predict that the purification experiment would fail ?
Hi Michelle, what were some of your controls for this experiment, just curious? Also, did you predict that your purification experiment would fail?
Hey Michelle, could you further explain what the significance of the plaque sizes were and how you determined whether or not it was significant research?
Hello Kirsten,
The purification experiment is where we scraped a single plaque from the enrichment plate and incubated it in phage buffer to enrich that single plaque. We did this twice in order to make sure we had a single phage specie. The purification experiment may have failed because the plaque we used was a relatively big plaque, so it may have had two species of phage.
Hello Sara,
Yes it is from both.
Hello Luis,
We were able to tell that it was two different phages from the size of the plaques.
Hello Ray,
We didn’t really have any controls since the experiments we conducted were mostly characterization. We didn’t expect for the purification experiment to have failed actually. But, we did hypothesize that the phage was highly infectious in mycobacterium because we always ended up in with cleared plate.
Hello Salma,
It’s important that we recognize plaque size because each different phage species, when infecting bacterium, will result in a different plaque size. That is how we were able to deduce that we had two different phages because there were two different sized plaques.