Hi Fayth! 🙂 Good job on your presentation. You mentioned you had some contamination in your results, what do you think this could be from? What kind of contamination would you expect in this case?
In our restriction digest we use CutSmart buffer to help aid the cutting of the phage DNA. Because there are not clear bands in our results, we assumed the CutSmart was contaminated and wasn’t able to perform those cuts. The other groups using the gel we used also had unsatisfactory results, and with some discussion with our TA Ashleigh we were not able to come to a conclusive answer as to what may have been the contaminate.
Great question! A spot test is an experiment that can help answer a series of questions. In this case we used a spot test with dilutions of our High Titer Lysate, containing the DNA of our phage. The dilutions are placed 1-8 on an L-agar plate. The plaques that are present shows just how concentrated our lysate is!
That is a good question! During our experiments we are able to find out which lifecycle our phage goes through by identifying the morphology of the plaques. This can then later, in combination with a successful restriction digest and PCR, can tell us what cluster the phage belongs to. Then we can work toward characterizing it!
Great poster and presentation! Why did you adopt your phage from another group?
My lab group was not able to get any phage out of the enrichment process from our soil samples, so we adopted in order to continue research.
Hi Fayth! 🙂 Good job on your presentation. You mentioned you had some contamination in your results, what do you think this could be from? What kind of contamination would you expect in this case?
In our restriction digest we use CutSmart buffer to help aid the cutting of the phage DNA. Because there are not clear bands in our results, we assumed the CutSmart was contaminated and wasn’t able to perform those cuts. The other groups using the gel we used also had unsatisfactory results, and with some discussion with our TA Ashleigh we were not able to come to a conclusive answer as to what may have been the contaminate.
Good job!! How does a spot check help in your research? What exactly is it and what conclusion did it allow you to draw?
Great question! A spot test is an experiment that can help answer a series of questions. In this case we used a spot test with dilutions of our High Titer Lysate, containing the DNA of our phage. The dilutions are placed 1-8 on an L-agar plate. The plaques that are present shows just how concentrated our lysate is!
What is the importance of the two different lifecycles of phages in the context of your experiments?
That is a good question! During our experiments we are able to find out which lifecycle our phage goes through by identifying the morphology of the plaques. This can then later, in combination with a successful restriction digest and PCR, can tell us what cluster the phage belongs to. Then we can work toward characterizing it!
Nice presentation Fayth! What does the EM photo of your phage tell you about its characterization?