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10 thoughts on “P43 – Keshk”
Did the EM picture help determine the cluster?
Can phages be harmful if we put them in their body?
No, the EM picture only gave us insight as to the morphology of the phage; so just whether it was a siphoviridae or pidoviridae or another type.
They can be. Your immune system can recognize it as a foreign body and may try to attack it. If you use the proper type of phage, then they shouldn’t infect the good bacteria in your body.
What were your expected results? I’m not sure what you were supposed to see vs what you actually got.
Honestly, not much. I didn’t really expect a specific result because it was just discovering and characterizing a new phage.
I noticed you have a 10^-3 plate pictured and that there is still significant bacterial growth present on it. When I took this course I had a similar number of plaques on my 10^-8 plate and with completely clear 10^-3 and 10^-4 plates similarly to many other people in my class. Do you have any thoughts about why your results appear less impressive? Perhaps your titer was not well concentrated, for example?
Yes, you are correct. Our lysate wasn’t very concentrated. My partner and I though it was due to our phage not having a high replication rate. This was common in my class as well. Only a one or two people went up to 10^-8 dilutions. Now I am curious why that is.
What other experiments would you do if you had more time to get some more conclusive results for your phage?
I would definitely do another PCR because ours was pretty inconclusive. As well as sequencing the genome to be sure what cluster it belongs to.
Excellent presentation Mohammad!! You were fantastic all throughout the semester and such a thoughtful and hardworking learner! So glad to see that expressed in this video! Also just to answer the question you and Jenna were commenting on it most likely is due to a weaker infection rate with your pahge. Temperature plays a role and spring semester often isolates more phages and more infectious phages. The range at which phages are able to infect varies, and sometimes M. Smeg is not the best bacteria to isolate your phage on
Thank you for the response. I enjoyed our conversations during the online labs. That is interesting to know. I guess phage develop differently in a warmer climate.