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7 thoughts on “P47 – Duncan”
What is a plaque?
A plaque is a clear area field of bacteria on the plate that indicates the inhibition of the bacterial cells by some agent, in this case a bacteriophage.
How was the PCR gel used in relation to this research?
A PCR is completed after the Restriction Analysis, which we have yet to complete. But the restriction analysis will allow us to chose the cluster primers to use with our phage. After the PCR is completed we will run the samples on .8% agarose gel. The PCR will help allow us to determine the cluster and sub-cluster of our phage.
A PCR is completed after we complete the Restriction Analysis (which we have yet to get to) and have determined the cluster primer to use with our phage. The PCR is done to further help us identify the cluster and sub-cluster of our phage. This is important because we can then compare our phage to other phages in its’ sub-cluster and determine target strains of bacteria.
You mentioned a quality control gel in your future directions. How is this quality control gel important to identifying if your phage can be used in phage therapy?
A quality control gel is done after DNA isolation. The quality control gel is done to determine if our isolated DNA is of high enough quality to continue with further experiments.