7 thoughts on “P3 – Gilman

    1. There are multiple methods, such as orally, nasally, and IV. There are some risks, especially if not enough research is done beforehand. For example, the titer is important so that the immune system does not go into shock at the amount of foreign substance in the body but there must be enough to find and kill the bacteria. It is also important to know if the phage has tail fibers that could bind the receptors of good bacteria in the body and attack those bacteria instead.

  1. Because you identified your phage as temperate, does this make it any less effective in killing bacteria than a lytic phage?

    1. Yes, temperate phages still kill the bacteria, but only if they enter the lysing cycle. Since the temperate phages are able to enter both the lysogenic and lytic cycle, any phage that enter into the lysogenic cycle will not kill the bacteria until the phage feel it is necessary to enter the lytic cycle due to poor conditions in the cell. Since there is no guarantee of when or if the phage will enter the lytic cycle, it may not kill the bacteria or may take some time to do so, therefore temperate phages less effective at killing bacteria.

  2. This was a good presentation! You said for your future directions for this experiment include sequencing the phage to provide information that would characterize unique genetic markers for the phage’s application. What would this future application entail?

    1. The phage could be used for several different research studies. This phage could be used in a possible phage cocktail to help cure a disease and kill the bacteria in someone’s body. It could also be used to discover more similarly related phages to M. smeg and M. tuberculosis. People may also be able to use this phage to progress their own research.

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