Hey, nice presentation! I was wondering if you could explain the subclusters A2, A9, and B3 a little more? I’m unfamiliar with phages and what that means, if it refers to their anatomy or function or something similar. Thank you!
Essentially, a cluster or subcluster refers to how similar the phage genome is compared to other phages. To be placed into a cluster, it must have at least 50% similarity and it needs 70% similarity to be placed into a subcluster! So the phage we had discovered, displayed similarity in those subclusters and a PCR will be run to either confirm or deny that.
I really liked your presentation, and it was super interesting to hear it from the perspective of someone who actually did the lab. I did half and gave up so I know a little bit about it. I am just curious to know how successful you were in finding a phage in your dirt samples? Thank you!
Hi! I really liked your presentation and loved hearing about your phage results. I was just wondering how successful you were at finding your phages in the dirt the first few times? And when they were identified how many clusters did you have? Thank you!
We had a lot of success! We were able to isolate just one phage without having to repeat any isolation or enrichment experiments. When we had identified we had a phage, there were a couple clusters that showed a wide range of similarity but clusters A and B showed the most similarity.
The plaque size has no clear indication on whether it is lytic or temperate. But, with other plaque morphology, such as the rapid growth of small, clear plaques throughout the plate, it led me to infer that the phage that we had isolated was lytic.
Hey, nice presentation! I was wondering if you could explain the subclusters A2, A9, and B3 a little more? I’m unfamiliar with phages and what that means, if it refers to their anatomy or function or something similar. Thank you!
Essentially, a cluster or subcluster refers to how similar the phage genome is compared to other phages. To be placed into a cluster, it must have at least 50% similarity and it needs 70% similarity to be placed into a subcluster! So the phage we had discovered, displayed similarity in those subclusters and a PCR will be run to either confirm or deny that.
I really liked your presentation, and it was super interesting to hear it from the perspective of someone who actually did the lab. I did half and gave up so I know a little bit about it. I am just curious to know how successful you were in finding a phage in your dirt samples? Thank you!
Oops that posted twice. Sorry!
Hi! I really liked your presentation and loved hearing about your phage results. I was just wondering how successful you were at finding your phages in the dirt the first few times? And when they were identified how many clusters did you have? Thank you!
We had a lot of success! We were able to isolate just one phage without having to repeat any isolation or enrichment experiments. When we had identified we had a phage, there were a couple clusters that showed a wide range of similarity but clusters A and B showed the most similarity.
This was a very informative presentation! I was wondering why the plaque size of <0.5mm would indicate that the cycle is lytic. Thank you!
The plaque size has no clear indication on whether it is lytic or temperate. But, with other plaque morphology, such as the rapid growth of small, clear plaques throughout the plate, it led me to infer that the phage that we had isolated was lytic.