The different clusters and subclusters have very similar genomes to each other, and could possibly lead to discovering more about the phage and what host bacteria it could infect.
The plaques have clouded edges and clear center which led us to the conclusion that it is temperate. Temperate phages can undergo both of the lifecycles, and when the phage is in the lysogenic lifecycle the bacteria in the spot is not all killed, but some of it is which makes the cloudy edges of the plaque.
We did many dilution series. the first 2 rounds of dilution were for purification. This was so that we could isolate one specific phage in case the soil sample we collected had multiple different phage in it. After we purified it we did another round of dilutions in order to figure out the titer or concentration of phage in our lysate and to make a high titer lysate for DNA isolation.
It could be used in a phage cocktail if the specific conditions for it to only undergo the lytic lifecycle were found and met. Unfortunately, they are not super likely to be used in medicine since they don’t always lyse the bacteria and because the integration of the genome can make the host bacteria non-susceptible to other phage or treatment.
Hello Rachel! What does it mean to be an A1 subcluster?
The different clusters and subclusters have very similar genomes to each other, and could possibly lead to discovering more about the phage and what host bacteria it could infect.
What in the plaque morphology leads you to conclude your phage is temperate and why does it apply?
The plaques have clouded edges and clear center which led us to the conclusion that it is temperate. Temperate phages can undergo both of the lifecycles, and when the phage is in the lysogenic lifecycle the bacteria in the spot is not all killed, but some of it is which makes the cloudy edges of the plaque.
What was the reason for doing a dilution series? And what does it mean to be a Siphoviridae morphotype?
We did many dilution series. the first 2 rounds of dilution were for purification. This was so that we could isolate one specific phage in case the soil sample we collected had multiple different phage in it. After we purified it we did another round of dilutions in order to figure out the titer or concentration of phage in our lysate and to make a high titer lysate for DNA isolation.
The siphoviridae morphotype is characterized by the very long tail as seen on the EM image.
Is there some significant characteristic of the A1 type phages that differentiates them from other types?
What medical purposes could the temperate phage be used for specifically?
It could be used in a phage cocktail if the specific conditions for it to only undergo the lytic lifecycle were found and met. Unfortunately, they are not super likely to be used in medicine since they don’t always lyse the bacteria and because the integration of the genome can make the host bacteria non-susceptible to other phage or treatment.