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8 thoughts on “P67 – Mendik”
How did you perform your serial dillution?
Our phage lysate was diluted to five different dilutions in phage buffer. Then each dilution was grown on an agar plate in M. Smeg. For our experiment, only the 10^0 plate showed a web pattern.
In your poster, you mention how it’s a good thing your lytic phage has a high burst rate. Why specifically is that a beneficial characteristic to have when looking for a phage that could possibly be used in medical research to fight bacterial infections?
It would be more efficient at infecting other nearby bacteria cells since the phages could reach farther during the burst.
You mention that the phage is a lytic phage, but I’m wondering how that is determined? Just by size and shape and clear defined edges alone?
It was theorized to be lytic based off the morphology. The plaques consistently had clear, defined edges which is a major characteristic of lytic phages.
What were you looking for when selecting plaques to study? Were there certain characteristics you were looking for when choosing plaque A or plaque B?
We were looking for spots that were most likely plaques. The original enrichment isolation had a clear bacterial lawn, however it was unclear if some spots were impurities in the gel or plaques. The first spot A did not produce any plaques on the streak assay. The spot B did produce plaques. It was partially looking for clear spots and partially just trying it on a streak assay.