As I mentioned, we need to do PCR as a step towards confirming the cluster identity. My lab partner used the restriction digest to generate a different cluster hypothesis and believed that NastyNellie was a B cluster phage because she used Phage Enzymes Tools in a slightly different way. So we then performed PCR using B4 cluster primers, but since NastyNellie is not in that cluster it yielded no results.
Our phage was temperate, which means that it does undergo both the lytic and lysogenic lifecycles. We can see that it is temperate from the plaque morphology with the lytic zone surrounded by the turbid outer ring. The outer ring results from phage DNA integrating into the host genome (lysogenic lifecycle) and creating a plasmid, which is then unable to be infected by other phage DNA.
curesymposium.org 
You said that you had done other experiments what were those experiments?
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As I mentioned, we need to do PCR as a step towards confirming the cluster identity. My lab partner used the restriction digest to generate a different cluster hypothesis and believed that NastyNellie was a B cluster phage because she used Phage Enzymes Tools in a slightly different way. So we then performed PCR using B4 cluster primers, but since NastyNellie is not in that cluster it yielded no results.
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If your phage had ended up being lysogenic, what would you expect to change within your results/ experiments.
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Our phage was temperate, which means that it does undergo both the lytic and lysogenic lifecycles. We can see that it is temperate from the plaque morphology with the lytic zone surrounded by the turbid outer ring. The outer ring results from phage DNA integrating into the host genome (lysogenic lifecycle) and creating a plasmid, which is then unable to be infected by other phage DNA.
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I believe I may have said plasmid, I meant prophage:)
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Do you happen to know how long temperate phages can stay dormant inside the host cell?
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Hi Ava! What would you say was your biggest challenge when conducting this experiment?
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