You determined that the Tempura phage was a BU cluster phage and siphoviridae, but what kind of classifications are these? Does it indicate that it may infect a certain genus of bacteria etc?
The siphoviridae is the family that the phage belongs to and its determined by the shape/ratio of the phage. Clusters are just a way to categorize the phage based on their genome similarity.
Siphoviridae is the family that the phage belongs to and is determined by the shape/ratio of the head to tail length. Clusters are another way to group phages and phages are grouped in cluster by the similarity of their genomes. Those classifications don’t necessarily determine which bacteria the phage can infect because that’s determined by a gene but phage in the same cluster/subcluster have a higher chance of infecting the same bacteria because their genes are similar.
Clusters are another way to organize the phage into groups and phage inside the same cluster have a similar genome, so this means that Tempura’s genome is similar to the genomes of other phages inside the BU cluster.
A lot of trials have to be done before the phage therapy is done on a human and those trials include trials like we did in this experiment and also animal testing. Once it has gone through those trials, the phage cocktail(which includes the phage that were tested on in the trials) will be accurately dosed for the patient and then is typically administered through an IV.
What was the reason for naming this phage “Tempura”?
There’s no real reason other than my partner and I both love sushi and she threw out the idea.
You determined that the Tempura phage was a BU cluster phage and siphoviridae, but what kind of classifications are these? Does it indicate that it may infect a certain genus of bacteria etc?
The siphoviridae is the family that the phage belongs to and its determined by the shape/ratio of the phage. Clusters are just a way to categorize the phage based on their genome similarity.
Siphoviridae is the family that the phage belongs to and is determined by the shape/ratio of the head to tail length. Clusters are another way to group phages and phages are grouped in cluster by the similarity of their genomes. Those classifications don’t necessarily determine which bacteria the phage can infect because that’s determined by a gene but phage in the same cluster/subcluster have a higher chance of infecting the same bacteria because their genes are similar.
What exactly is a BU cluster phage and what can we benefit from identifying that?
Clusters are another way to organize phage and Tempura being a BU cluster phage means that its genome is similar to the other BU cluster genomes.
Clusters are another way to organize the phage into groups and phage inside the same cluster have a similar genome, so this means that Tempura’s genome is similar to the genomes of other phages inside the BU cluster.
What exactly does Phage therapy entail?
A lot of trials have to be done before the phage therapy is done on a human and those trials include trials like we did in this experiment and also animal testing. Once it has gone through those trials, the phage cocktail(which includes the phage that were tested on in the trials) will be accurately dosed for the patient and then is typically administered through an IV.
what does phage therapy entail?