8 thoughts on “P39 – Whitefield

    1. Great question! Some potential impacts are the use of phage therapy, the phage I isolated is temperate so it would need to be mutated in order to undergo the lytic cycle to kill bacteria. Once it has been mutated it has the potential to kill bacteria genetically similar, most notably M.tuberculosis which causes tuberculosis in humans.

    1. Great question! Temperate phage have the ability to undergo the lysogenic and lytic lifecycle, while the lytic cycle is when lysis of the cell occurs, in order for my phage to be effective in killing bacteria it must undergo the lytic cycle. If it was just used in it’s temperate state it might not kill the bacteria in a patient, where as if it were mutated it would be sure to kill the infection.

    2. My phage would need to be mutated in order to be effective in phage therapy. With my phage being temperate it has the ability to undergo either lytic or lysogenic lifecycles, this means it has the potential to not kill the bacteria which would be ineffective in phage therapy.

  1. You mention your phage belonged to the Siphoviridae family, do you know much about this family and is there anything special or unique about them? I also love your phages name 😀

    1. Great question! There is a wide variety of families phage can belong to but the main ones are siphoviridae and myoviridae, siphoviridae is characterized by a long non-contractile tail and myoviridae have short contractile tails, this difference is key in how the phage inserts it DNA into a bacterial host cell, with a contractile tail the phage can pierce the host cell and insert it’s DNA while with a non-contractile tail and enzyme is used to break down the bacterial cell wall in order for the phage to insert it’s DNA.

  2. Hi! Great presentation! You were able to categorize your phages in to different groups and I was just wondering what the differing characteristics are between the Siphoviridae family and the L cluster?

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