Identifying phages with similar genetic sequences allows us to classify our phage into a cluster of phages that are similar which is helpful in phage therapy because phages in the same cluster are more likely to have similar host ranges(range of bacteria a bacteriophage can infect) and this allows us to be more precise and methodical when testing bacteriophages on certain species of bacteria instead of making it random.
I’m assuming you mean the DNA. Phage DNA can be isolated by taking the phage lysate and mixing in DNA resin and nuclease mix. You then add EDTA which helps to neutralize the nuclease mix which leaves a DNA clean up resin which can be filtered by pouring isopropanol a few times through DNA wizard columns containing the DNA clean up resin in order to wash the DNA. The DNA wizard columns are centrifuged a few times in order to purify and remove any particles left in the DNA and then you’re left with just DNA in the DNA wizard columns.
A possible advantage temperate phage have over lytic phage is that because they integrate within the host’s genome, they are able to survive for a long period of time and then change into the lytic life cycle once the environmental conditions inside are unfavorable.
Great presentation, though I am curious what the purpose of identifying phages with similar genetic sequences would be.
Identifying phages with similar genetic sequences allows us to classify our phage into a cluster of phages that are similar which is helpful in phage therapy because phages in the same cluster are more likely to have similar host ranges(range of bacteria a bacteriophage can infect) and this allows us to be more precise and methodical when testing bacteriophages on certain species of bacteria instead of making it random.
How do you isolate the bacteria of the phage?
how do you isolate the bacteria of a phage? (around 2:30 mark you mention it)
I’m assuming you mean the DNA. Phage DNA can be isolated by taking the phage lysate and mixing in DNA resin and nuclease mix. You then add EDTA which helps to neutralize the nuclease mix which leaves a DNA clean up resin which can be filtered by pouring isopropanol a few times through DNA wizard columns containing the DNA clean up resin in order to wash the DNA. The DNA wizard columns are centrifuged a few times in order to purify and remove any particles left in the DNA and then you’re left with just DNA in the DNA wizard columns.
What could some advantages be for using temperate phage compared to lytic phage in the sense of medicine?
A possible advantage temperate phage have over lytic phage is that because they integrate within the host’s genome, they are able to survive for a long period of time and then change into the lytic life cycle once the environmental conditions inside are unfavorable.