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12 thoughts on “P75 – Brockman”
Good presentation! I was just wondering did you and your partner run into any complications during the experiments?
Thank you for the question. We had no issues isolating our phage other than having to repeat the serial dilution to dilute the lysate to a point that we had a countable number of plaques on a plate. after our quality control gel we ran a restriction digest that failed.
At 3:04 in your presentation, what do you mean that your phages had “positive quality?” Does this mean that the images were good quality or is it describing the quality of the phage itself?
Sorry about the confusion, all I meant by positive quality is that there was DNA isolated and that it was of decent quality.
what are the real-world impacts of this research?
Good question. Phages that are lytic can be an alternative to an alternative to antibiotics, especially these days as antibiotic resistance bacteria become more prevalent.
As a student not in phage lab, I would like to know how the size of the phage differs between the different types of phages. You state your phage length, but what does that mean?
Great question, this is a question that needs more research done. Phages can have several different types of tails and heads, this is currently more of a way of categorizing the phage and helping to determine cluster. But more research needs to be done into how the sizes of the heads and tails effect the phage.
Does plaque size have anything to do with the morphology of your phage ?
Thank for the question. The plaque size has to do with the infection rate of phage. Lytic phages tend to have smaller plaques since the cause immediate cell death to all infected bacteria cells.
If and/or when you do compare your phage to other phages of the same cluster, what genes are you expecting to see in similar phages? What genes do you think will be common?
Great question, the main genes I would think would be similar would be the capsid and tape measure genes, as well, possibly lysin and other genes responsible for lysis.