11 thoughts on “P45 – Daniels

  1. Did you run into, or do you predict future experiments will run into any roadblocks while looking further into this research?

    1. When looking at future experiments, I think something that would cause a roadblock is being able to combine this phage with other phages in a phage cocktail for phage therapy since it is in such a small niche cluster. Most future experiments are to confirm the cluster hypothesis, but it is pretty evident that it belongs in the O cluster.

  2. Hi Paige, great job! You mentioned wanting to sequence the genome of your phage as a future direction, I am wondering how difficult this process is with regard to phages and how it might help future research? Would it be just to identify what other bacteria the phage could infect?

    1. Thank you! The process of isolating the phages is not as challenging compared to the rest of the experiments. The bacteria the phage infected in our experiments is similar to the bacteria of Tuberculosis, so this would be used to help along the lines of that instead of completely different bacteria. With regards to future research, this can help combat the battle against uprising antibiotic resistance.

      1. Also, genome sequencing is to help determine the proteins within the phage and their functions. This is to further help identifying a cluster and it is not difficult to achieve.

  3. In your poster, you mention that a “corndog” shape is characteristic of mycobacteriophages within the O cluster. Were there any other characteristics that you saw that allowed you to infer / confirm this identification of it being in the O cluster (IE overall size, tail shape, etc.)?

    1. Yes, the O cluster happens to be a rare form of phage, so it is easy to characterize and put it into the O cluster due to the fact that it has the oval like head and long tail that is recognizable of the Siphoviridae family.

  4. What do you plan to achieve if you finish your PCR correctly as stated in your future directions? Also would you plan to see anything unusual when doing so?

    1. With regards to the PCR test, we plan to achieve results that further prove and solidify that the phage belongs in the O cluster. Since most of our experiments came out proving no abnormalities with regards to cluster hypothesis, I do not see a reason for anything unusual to come out of the PCR.

  5. What is an O cluster? I am a phage genomics laymen and I got confused by some of the terms for sure.

    1. The O cluster is a category within the Siphoviridae family. This is used to categorize phages in order to make them easier to find when selecting for a phage cocktail in phage therapy. The O cluster happens to be one of the more uncommon clusters as referred to in the presentation.

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