I wish that we were able to do our own plating of the lysate when trying to calculate the titer of the lysate. We had to let the TA’s do the plating due to covid campus closures and because of this it was contaminated and we were never able to decontaminate it so we could not archive it.
We would use the overall phage genome to compare it to other phages with similar genomes and see if they have a specific use that was already found. If so that could give us indication to potential uses for our phage.
Why were the bands under EcoRI and HindIII so bright?
They were very bright because there was a large amount of cuts that were bigger than 10 KB.
What is one thing you wish that you did differently?
I wish that we were able to do our own plating of the lysate when trying to calculate the titer of the lysate. We had to let the TA’s do the plating due to covid campus closures and because of this it was contaminated and we were never able to decontaminate it so we could not archive it.
How would you use your phages genome to compare it to other phages? RE’s, TF’s or just the overall genome?
We would use the overall phage genome to compare it to other phages with similar genomes and see if they have a specific use that was already found. If so that could give us indication to potential uses for our phage.