8 thoughts on “P47 – Duffy

  1. Great job on your presentation! You are clearly knowledgable and were well-spoken! My question is: What would be the procedure if the results in figure 4 were shown to be unfavorable? I.e. no band and the presence of degradation

    1. Hi, thanks for your question!
      If there was no band or if degradation was present, then another quality control gel would be run.
      If repeated quality control gels still resulted in no bands or degradation, then DNA isolation would be conducted again for more quality DNA to be run.

  2. You are very knowledgeable and very well spoken! Great job presenting your poster! Just a quick question about how bacteriophages become present in soil and if they are centralized to one location in the environment or if they can be found at multiple sample sites (ie: mountain soil vs city soil)?

    1. Thanks for your question!
      Bacteriophages can be found at multiple sample sites, including both mountain and city soil and just about anywhere. They’re more likely to be found in the same environment as their bacterial host.

  3. Good job! As an add on to the question above, if the results from figure 4 were unfavorable, does that mean that Kayen is not a candidate for further research within a host?

    1. Thanks for your question!
      Yes, if there wasn’t enough DNA present, or it wasn’t of quality then the rest of the experiments such as restriction digest could not be conducted, as it would be hard to further determine results.

  4. hi nice job! “Determine the host range of Kayen through spot testing Kayen’s lysate
    onto other bacterial strains” can you explain this a little bit further?

  5. Thank you!
    The host range are the types/ strains that bacteriophage can kill efficiently. Determining the host ranges are important for phage therapy, because it helps researchers know what phages they can use to kill certain bacteria. In the case of phage cocktails in phage therapy, multiple bacteriophages can be combined and administered, resulting in more effective lysing (killing) of the bacteria, because they have a wider host range. The host range of bacteriophages can first be determined by taking their lysate (concentrated phage in liquid form) and spot testing it onto different bacteria strains. Spot tests consist of different concentrations of lysate administered onto a lawn of the bacterial strain on a plate. Then, researchers can observe if the bacteriophage efficiently lysed the host strain on the plates. We would essentially do this with Kayen to determine its host strain.

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