8 thoughts on “P86 – Hoyt

  1. The presentation was great. You mentioned using phage in order to treat bacterial infections as they would lyse the bacteria. Do you think that this could work equally as well for your temperate phage?

    1. Great question! Lytic phages lyse the bacteria and kill the bacterial cell and therefore can be used in bacterial infections. However, because the phage I isolated was temperate, which will not lyse the cell, I would have to genetically modify my temperate phage to remove the repressor in order for the temperate phage to be successful in killing bacterial cells.

  2. What kinds of future directions could you give in terms of how your phage could be used in further research/treatment?
    Could it be used to lyse bacteria to remedy antibiotic resistant infections?

    1. Future directions that could be given for further research for my temperate phage would be to insert a fluorescent marker into the phage genome which when it infects a bacterial cell, for example E. coli, it would fluoresce and be a preventative measure in the distribution of produce. Because my phage has a repressor present it would not be able to lyse the bacterial cell, instead it would insert itself into the bacterial genome. However, I could use my phage in a cocktail of phages if I genetically modify my phage to become lytic in order to treat antibiotic resistant infections.

    1. Great question! So actually we did not chose our phage. We used enrichment from a soil sample we collected from Boulder, CO and introduced the phages in that sample to a mycobacterium M. smegmatis. Over multiple plaque streaks we were able to isolate one specific plaque and collect DNA, and a high titer lysate and analyze that specific phage. The result from our findings were that we had collected a temperate phage, due to the cloudy plaques and halos, with a siphoviridae morphology. .

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