How are the plaques assessed to determine whether a phage is lytic or not? Is any plaque above a specific size threshold considered to have been lysed?
Lytic phages present as circular and clear, whereas turbid (plaques that underwent lysogeny) are cloudy. Lytic phages are clear because they completely lyse/kill the bacteria. Cloudy plaques contain a prophage, and integrate themselves in the host bacteria. They do not completely kill the host bacteria. Instead, lysogens from in the center of the plaque which often form a bullseye turbid plaque.The size does not necessarily directly determine whether it is lytic or not. Instead, the morphology indicates lytic vs temperate!
What is the significance of your results? In other words, since bateriophage chula has high quality DNA what does this mean for future testing! Very interesting experiment!
Thank you for your comment! Since the quality control gel indicated that Chula had quality enough DNA, that means we would be able to proceed and perform a restriction digest to determine what cluster it belongs to. We could then perform a PCR test to confirm what we gathered from the restriction digest. If Chula’s DNA did not present as “quality enough” on the gel, we would have to redo previous experiments until we have DNA that could be used for future experiments.
Hi Emily, I really like your poster’s organization! Even though there is a general format to follow, the spacing and clear separation of sections made the poster very easy to follow while listening to your presentation.
In terms of clinical use, what are some of the limitations or lack thereof in treating human infections with bacteriophage?
Thank you! In terms of clinical use, phages have the potential of triggering an imbalance or overreaction in the immune system. In addition, some phages have the potential of causing bacteria to become resistant to certain clusters. It can also sometimes be difficult to figure out which phage is most effective for treating certain infections.
In this presentation you make a distinction between lytic and lysogenic morphology. Are these two types of phages or are they two states that a single phage can be in?
How are the plaques assessed to determine whether a phage is lytic or not? Is any plaque above a specific size threshold considered to have been lysed?
Lytic phages present as circular and clear, whereas turbid (plaques that underwent lysogeny) are cloudy. Lytic phages are clear because they completely lyse/kill the bacteria. Cloudy plaques contain a prophage, and integrate themselves in the host bacteria. They do not completely kill the host bacteria. Instead, lysogens from in the center of the plaque which often form a bullseye turbid plaque.The size does not necessarily directly determine whether it is lytic or not. Instead, the morphology indicates lytic vs temperate!
What is the significance of your results? In other words, since bateriophage chula has high quality DNA what does this mean for future testing! Very interesting experiment!
Thank you for your comment! Since the quality control gel indicated that Chula had quality enough DNA, that means we would be able to proceed and perform a restriction digest to determine what cluster it belongs to. We could then perform a PCR test to confirm what we gathered from the restriction digest. If Chula’s DNA did not present as “quality enough” on the gel, we would have to redo previous experiments until we have DNA that could be used for future experiments.
Hi Emily, I really like your poster’s organization! Even though there is a general format to follow, the spacing and clear separation of sections made the poster very easy to follow while listening to your presentation.
In terms of clinical use, what are some of the limitations or lack thereof in treating human infections with bacteriophage?
Thank you! In terms of clinical use, phages have the potential of triggering an imbalance or overreaction in the immune system. In addition, some phages have the potential of causing bacteria to become resistant to certain clusters. It can also sometimes be difficult to figure out which phage is most effective for treating certain infections.
In this presentation you make a distinction between lytic and lysogenic morphology. Are these two types of phages or are they two states that a single phage can be in?
The terms lytic and lysogenic refer to two lifecycles that phages can undergo.