Could your phage help aid research into how phage and bacteria are interacting in the gut microbiome and if so do you have thoughts on how it could do so?
It could do so by being sequenced and saved in the lab so once tests are to be ran and phases are needed in that specific area. They could take the phage from the lab and utilize it in tests and experiments
What information do you think you could gain from performing an electron microscopy of your phage? Would doing so have any benefits for your research and/or the understanding of phages?
Running the electron microscopy will give us a look at what our phage looks like. Specifically looking at the head and tail. With this information it can be clearly compared to other phages in its cluster or sub cluster. This would give more understanding to the research of what phages look like. I read an article on features based on cluster and sub cluster, it went into detail about how certain clusters or sub clusters have their own unique feature. This could be added more into research like that as well.
The lysate that we made from the web pattern plate is then used in the DNA isolation that is done. This is because in that lysate is our isolated phage and the work that they have been doing. These phages that were on that web pattern plate are in that lysate now so we can pull the DNA from that phage.
Nice job! What are the potential uses for lytic phages? I know that lysogenic phages are much more useful in terms of things like phage therapy, but I do not know much about the potential benefits to using lytic phages.
Lytic phages are more useful in determining fast acting work. When they go in they specifically start making more phages that then attack the virus. So for say fighting off a virus it’s almost better to use these lytic phages as they go in and take out the virus right away. Whereas temperate phages go into either or phase which can be less useful in its own sense.
Do you have an idea of what Cluster or sub clusters you would use when you get to the point of being ready to run a PCR?
At this point no we do not know cluster or subs cluster we will know that info once we run out restriction digest and get those results.
Could your phage help aid research into how phage and bacteria are interacting in the gut microbiome and if so do you have thoughts on how it could do so?
It could do so by being sequenced and saved in the lab so once tests are to be ran and phases are needed in that specific area. They could take the phage from the lab and utilize it in tests and experiments
What information do you think you could gain from performing an electron microscopy of your phage? Would doing so have any benefits for your research and/or the understanding of phages?
Running the electron microscopy will give us a look at what our phage looks like. Specifically looking at the head and tail. With this information it can be clearly compared to other phages in its cluster or sub cluster. This would give more understanding to the research of what phages look like. I read an article on features based on cluster and sub cluster, it went into detail about how certain clusters or sub clusters have their own unique feature. This could be added more into research like that as well.
In the second plaque, when you are isolation DNA, why is it important that you specifically develop a lysate plate to help identify DNA?
The lysate that we made from the web pattern plate is then used in the DNA isolation that is done. This is because in that lysate is our isolated phage and the work that they have been doing. These phages that were on that web pattern plate are in that lysate now so we can pull the DNA from that phage.
Nice job! What are the potential uses for lytic phages? I know that lysogenic phages are much more useful in terms of things like phage therapy, but I do not know much about the potential benefits to using lytic phages.
Lytic phages are more useful in determining fast acting work. When they go in they specifically start making more phages that then attack the virus. So for say fighting off a virus it’s almost better to use these lytic phages as they go in and take out the virus right away. Whereas temperate phages go into either or phase which can be less useful in its own sense.