6 thoughts on “P51 – Marikar

  1. Hey Nasreen, it’s so cool that you got your phage from a soil sample in Boulder! On the poster, you said that the phage successfully infected M. smegmatis, and that you want to see if it’s able to infect M. tuberculosis. What would be your next step, if it’s able to infect M. tuberculosis? If the end goal is to see how it works in people, how would you find out if it’s safe?

    1. If BigLiz (the phage) is able to infect M. Tuberculosis then I believe that it would somehow be tested on animal cells most likely through animal testing, or possibly in a more isolated manner. Human testing is also possible, but these sorts of trials would probably occur after animal testing.

    1. One type of plaque assay is when a sample of phage lysate is plated with the bacteria it is trying to infect on an L.Agar plate, and then incubated so that if the phage is able to infect the bacterial cells plaques will form after about 48 hours in incubation.

  2. Great project and very easy to follow! One question I have is how you would work towards your goal of testing in humans?

    1. Thank you! I think the process would begin with animal testing and then move to human trials. However, if there was a way to avoid animal testing I would be very interested in that method.

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