8 thoughts on “P96 – Baird

    1. Bacteriophage co evolve with their their host bacteria. These bacteria have evolved with M. smeg and in that process developed long non contractile tails that were most effective at infecting and in our case lysing M. smeg. If our specific bacteria had different tails that it did not evolve with it is likely that it would not be able to infect M. smeg.

  1. Why are you conducting this experiment? Why should someone conduct further research on your experiment?

    1. We conducted this experiment in hopes of isolating and characterizing a Mycobacteriophage. M. smeg is closely related to M. tuberculosis, so if our phage is capable of infecting M. smeg then it might be able to infect M. tuberculosis and be used in the treatment of antibiotic resistant tuberculosis.

  2. If you wanted to test the phage for its success with tuberculosis what would the first steps be?

    1. We would use a similar process. Provide a bacterial lawn of M. tuberculosis and a concentration of phage lysate would be introduced to said lawn. Depending on the results of this experiment the next steps would be decided.

  3. Great job on your presentation! Does the type of phage isolated affect the outcomes associated with phage therapy?

    1. Thank you! Yes absolutely, if the phage isolated is lytic (meaning it lysis’ or kills the host bacteria) rather than temperate (keeps the phage genome integrated in the host bacteria) than it is a much better candidate for phage therapy. We would not want to use a temperate phage for phage therapy because these phage do not kill the host bacteria and can actually make the host bacteria more resistant to infection of other phage.

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