Awesome presentation! I had two questions: What type of plaques to lysogenic bacteriophage produce, and how do you know?
Why is it important to make the distinction between a lytic and lysogenic bacteriophage?
Temperate phages produce cloudy/ turbid plaques. This is important because temperate phages integrate their DNA into the host cell, remaining dormant until environmental stressors cause the phage to lyse the cell whereas lytic phages go through the lytic lifecycle, immediately killing the cell. This impacter their possible uses for phage therapy.
Temperate phages create turbid, cloudy plaques. The difference between temperate and lytic phages alter how they can be used in phage therapy where one kills the bacterial cell while the other integrates its DNA into the host chromosome.
I would be looking for quality genes along with the length and size of the genome. Once the DNA is sequenced I would by looking at what genes are present if there are lysogenic genes or only lytic genes and if this matches the determination if the phage is lytic or temperate.
I would look at the the DNA along with the size/length. Once the DNA is sequenced I would then look at what genes are present if there are lytic genes and lysogenic genes and if this aligned with the determination of the phage being lytic.
Awesome presentation! I had two questions: What type of plaques to lysogenic bacteriophage produce, and how do you know?
Why is it important to make the distinction between a lytic and lysogenic bacteriophage?
Temperate phages produce cloudy/ turbid plaques. This is important because temperate phages integrate their DNA into the host cell, remaining dormant until environmental stressors cause the phage to lyse the cell whereas lytic phages go through the lytic lifecycle, immediately killing the cell. This impacter their possible uses for phage therapy.
Temperate phages create turbid, cloudy plaques. The difference between temperate and lytic phages alter how they can be used in phage therapy where one kills the bacterial cell while the other integrates its DNA into the host chromosome.
Hi Lauren! What would you be looking for in the DNA of your phage once you isolate it?
I would be looking for quality genes along with the length and size of the genome. Once the DNA is sequenced I would by looking at what genes are present if there are lysogenic genes or only lytic genes and if this matches the determination if the phage is lytic or temperate.
I would look at the the DNA along with the size/length. Once the DNA is sequenced I would then look at what genes are present if there are lytic genes and lysogenic genes and if this aligned with the determination of the phage being lytic.
Besides the bacterial strain which they were mentioned to infect, do you know of any other bacterial strains infected by this phage?
Currently, no, I do not know of any. I have not tested the host range yet or sequenced the phage.