6 thoughts on “S1 – Appleton

  1. Firstly, very cool. To take the soybeans that have been transfected would that be to then extract the casein proteins out of the soybeans rather than using the entirety of the soy bean?

    1. We would then grow it into a full plant so we could select for a germline with our milk proteins. We’d grow a whole plant but only extract the milk from the soybean itself.

  2. I thought this was extremely interesting! How is the extraction process of the soybean done exactly?

    1. First, we engineered our genes to have soybean export signals on them to be transported to the outer edge of the cell. Our cow milk proteins would be synthesized and transported to just behind the cell wall where we can then use a vacuum method to “squeeze” out the milk

  3. Super interesting! I had no idea micelles were in cow milk. How would you go about preventing the byproduct with opioid properties from being formed? Are these soybeans hard to care for? What kind of climate do they thrive in and do they have environmental consequences?

    1. We are preventing the opioid byproduct from being formed by using the coding sequence for the A2 Beta casein protein, which gets cleaved less often. Soybean are very easy to grow and care for, they also grow very quickly make them an ideal host organism for our milk product. Soybeans can pretty much be grown anywhere that has a warm season, water and sunlight. The environmental consequences would be much less than the current dairy industry.

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