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8 thoughts on “S2 – Kassanoff”
Why was E. Coli used as the model organism to grow them up?
E. Coli is easily grown in a lab and is able to replicate plasmids efficiently. Since it grows quickly, placing the vectors in E. Coli lets us obtain a greater number of plasmids for future use fairly easily.
Hey there! I really enjoyed your presentation. As far as your vector goes, would you say a vector and plasmid are the same? Is that an ampicillin resistant gene within the vector? I have worked with plasmids and E.coli so I am guessing so but wanted to make sure. Also, do you plan on continuing this research into the future to see how effective implanting this into soy beans would be? Great work!
Thank you for tuning in. The vector and plasmid are the same for these particular experiments, since the plasmid is used as the vehicle for the desired genes. All of the vectors we used had ampicillin resistance except crtE (which had a chloramphenicol resistance instead). This research will continue through the lab and hopefully will produce transformed soybeans in the future!
Why were soybeans chosen to place the Pacific Yew Tree gene in?
Soybeans grow much faster (~45-60 days) than the endangered Pacific Yew (~100 years), so they are more sustainable. In addition, soybeans are able to be cheaply cultivated on a large scale with current agricultural infrastructure.
When watching your video I was wondering if the poison taxol was similar to what y’all were studying?
Taxol/paclitaxel is thought to stabilize microtubules, preventing cell division, which leads to cell cycle arrest and cell death. For humans, in proper quantities, Taxol demonstrates anti-cancer properties. However, like many other current cancer treatments, Taxol is capable of killing healthy cells as well, which may be why you know it as a poison.