6 thoughts on “S5 – Carter

  1. If you were able to have gotten to inserting the plasmid into the soybean, did your plasmid have any promoter regions so that the proteins would be expressed? Or would they have been expressed using another promoter found in the soybean DNA?

    1. Hey Cameron!

      So we actually did not get our plasmid into a soybean yet, however, we do grow ups in E. coli bacteria. As for the promoter, this is a really interesting question because of the nature of our promoter. The E. coli grow ups did not produce our milk proteins because our promoter is allosteric, (side note, it is also a strong viral promoter). The allosteric part means that it has to be in the presence of a binding ligand in order to work. So in our case, the gene must be in the presence of ethanol to actually be promoted. The idea is that once in the soybean, the production of these proteins won’t mess up development of the plant and it will grow normally. If we want milk proteins, we put the beans in ethanol and just like that the gene will be promoted!

      Hope this helps,
      Jake Carter

  2. Did your plasmid have promoter regions so that the genes be expressed in the soybean once the DNA was infected into the soybean genome?

    1. Hey Cameron,

      I think you commented this question twice so I answered the more specific question.

      Thanks for the great question!
      Jake Carter

  3. In terms of the different versions of Casein with small differences in sequence, which is the most effective? Are there some that are more effective than others or does this method require all of the components to be put in use?

    1. Hey there Heidi!

      So the Casein proteins were in AS1, AS2, Kappa, and Beta conformations. The only one of those with 2 types were Beta (BA1, BA2). The reasoning for trying BA2 is because we found some literature on how BA1 is not digested as well by some humans. The rest of the proteins only had their 1 native conformation. Now for efficacy: the micelles I talked about have to have all 4 proteins present to form. If we just expressed kappa for example it would not be possible to form the micelle we are interested in. The micelle must have all 4 conformations present (and in the correct percentages) to form. It wouldn’t matter what version of beta we used for the purpose of the micelle though.

      Hope this answered your question!
      Jake Carter

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