We actually repeated the PCR twice and the results shown here are from the second round. Our gene specific primers maybe defective as they did not work in any of our PCRs, ideally if we could use new one we could get results.
Great question, a thermocycler cycles the temperature of the sample to induce amplification of our DNA product. The voltage affects the clarity of the gel images, we chose 120 volts because out PCR products were large.
In humans, Yes! But T.tetrahymena have a lot better DNA damage repair mechanisms and not all are clearly understood. Each group tested a different possible candidate gene for T.tetrahymena DNA damage repair.
This is a double strand break repair mechanism which is more difficult than a single strand break repair but is necessary in T.tetrahymena for recombination of its genome.
curesymposium.org 
Great job! Could you only perform one PCR procedure, or if you could perform another, what is something you would change to maybe get results?
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We actually repeated the PCR twice and the results shown here are from the second round. Our gene specific primers maybe defective as they did not work in any of our PCRs, ideally if we could use new one we could get results.
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I really enjoyed the presentation and poster! What is a thermocycler and how does the voltage affect the outcome?
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Great question, a thermocycler cycles the temperature of the sample to induce amplification of our DNA product. The voltage affects the clarity of the gel images, we chose 120 volts because out PCR products were large.
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Hi Mir! Great research topic. I was wondering if there are any known genes that repair DNA damage?
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In humans, Yes! But T.tetrahymena have a lot better DNA damage repair mechanisms and not all are clearly understood. Each group tested a different possible candidate gene for T.tetrahymena DNA damage repair.
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What kind of DNA repair process is this?
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This is a double strand break repair mechanism which is more difficult than a single strand break repair but is necessary in T.tetrahymena for recombination of its genome.
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Great presentation, how do you think the PCR experiment failed? If you were to redo it, what would you do differently to ensure an effective result?
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