thank you! were not quite sure why the gDNA was degraded, this was an issue that the entire class had. One idea is that the gDNA evaporated during PCR.
Thank you! We would have to do further testing, we would do this by testing different homologs through RNA seq and compare it to other genes. But its definitely a possibility.
Yes we did! we used quantitative measurements to confirm a higher expression of the Rad51 and Rpp0 +/-RT treated and untreated which showed that DNA damage was in fact induced. We also used measurements to see if the bands with the Hypedc3 gene and DNA damage which showed no increase in expression showing that the gene did not play a part in DNA damage response.
Great job on the presentation! What is Tetrahymena thermophila and why did you use it for this project? Do you think that information learned about DNA repair in this organism will be applicable in humans or lead to new experiments for learning about DNA repair in humans?
Thank you! The Tetrahymena was used cause it is a model organism, this is due to its rapid growth and its ability to cut its genome after every reproduction. Yes by fidning out the function of genes and proteins within the DNA damage response doctors and researchers can target these specific areas and figure out if the DNA damage response is not working properly, like in cancer and Huntigtons disease treatment, where issues in DNA damage occur.
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Great Presentation! What happens to the gDNA that causes it to degrade?
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thank you! were not quite sure why the gDNA was degraded, this was an issue that the entire class had. One idea is that the gDNA evaporated during PCR.
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Nice presentation! Do you think these specific DNA sequences would be a part of DNA damage repair in any other families?
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Thank you! We would have to do further testing, we would do this by testing different homologs through RNA seq and compare it to other genes. But its definitely a possibility.
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Great job! Did your group do any quantitative analysis to put numbers to the band intensity? What did those numbers show?
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Yes we did! we used quantitative measurements to confirm a higher expression of the Rad51 and Rpp0 +/-RT treated and untreated which showed that DNA damage was in fact induced. We also used measurements to see if the bands with the Hypedc3 gene and DNA damage which showed no increase in expression showing that the gene did not play a part in DNA damage response.
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You did such a good job! Do you have any proteins in mind that you would test within the same family?
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thank you! yes there are many in the thermophila family, and a variety in this same lab that could be used for further research. for example Rrm2
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Great job on the presentation! What is Tetrahymena thermophila and why did you use it for this project? Do you think that information learned about DNA repair in this organism will be applicable in humans or lead to new experiments for learning about DNA repair in humans?
LikeLike
Thank you! The Tetrahymena was used cause it is a model organism, this is due to its rapid growth and its ability to cut its genome after every reproduction. Yes by fidning out the function of genes and proteins within the DNA damage response doctors and researchers can target these specific areas and figure out if the DNA damage response is not working properly, like in cancer and Huntigtons disease treatment, where issues in DNA damage occur.
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