Hi! Could you provide more detail on why that model organism was used even though it is relatively unstudied? What is the benefit of using an organism with very little preexisting information on it? Thanks!
How does hydroxyurea induce damage to DNA? (does it cause denaturation? double or single-stranded breaks?) and does the presence of hydroxyurea affect proteins as well or is it able to only target and damage DNA?
Great question. Hydroxyurea, in the concentrations that we used, induces double strand breaks by depleting dNTP pools in the cell. Without dNTPs for the cell to use, replication processes stall and produce breaks. Assumedly, single strand breaks also occur.
Hello, can you further elaborate on what the different brightness of the bands of the AMN primers in figure 3 mean? I like that you used a computer program to quantify the difference in brightness.
The brightness of the AMN primer lanes in figure 3 correspond to the amount of mRNA expressed in each condition. The lane labelled HU was the one with DNA damage. UT was the one without induced DNA damage.
It was unknown to Suzanne Lee in the preceeding research what the function of these sRNAs was. The implication of sRNA’s involvement in break repair is that we’re able to know their function.
Regular PCR repeatedly cycle through DNA replication and all that is quantified is the end product. In q-PCR, the amount of DNA is measured after every cycle. Thus, q-PCR can let us see the true amounts that may be hindered by the limitations of PCR and gel electrophoresis. For instance, brightness values on a gel electrophoresis band could exceed the maximum output that can be read accurately.
This was a great presentation! Do you have any thoughts or predictions on which proteins RDN2 will interact with based on other research or the results you found during your experiments?
curesymposium.org 
Hi! Could you provide more detail on why that model organism was used even though it is relatively unstudied? What is the benefit of using an organism with very little preexisting information on it? Thanks!
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How does hydroxyurea induce damage to DNA? (does it cause denaturation? double or single-stranded breaks?) and does the presence of hydroxyurea affect proteins as well or is it able to only target and damage DNA?
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Great question. Hydroxyurea, in the concentrations that we used, induces double strand breaks by depleting dNTP pools in the cell. Without dNTPs for the cell to use, replication processes stall and produce breaks. Assumedly, single strand breaks also occur.
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What is cDNA and why did you use it in this experiment? How does it impact the experiment if cDNA is not used?
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Hello, can you further elaborate on what the different brightness of the bands of the AMN primers in figure 3 mean? I like that you used a computer program to quantify the difference in brightness.
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The brightness of the AMN primer lanes in figure 3 correspond to the amount of mRNA expressed in each condition. The lane labelled HU was the one with DNA damage. UT was the one without induced DNA damage.
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What are the implications if the sRNAs are involved in double strand break repair? Why does this matter?
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It was unknown to Suzanne Lee in the preceeding research what the function of these sRNAs was. The implication of sRNA’s involvement in break repair is that we’re able to know their function.
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What is the difference between the PCRs that you mentioned in your future directions?
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Regular PCR repeatedly cycle through DNA replication and all that is quantified is the end product. In q-PCR, the amount of DNA is measured after every cycle. Thus, q-PCR can let us see the true amounts that may be hindered by the limitations of PCR and gel electrophoresis. For instance, brightness values on a gel electrophoresis band could exceed the maximum output that can be read accurately.
LikeLike
This was a great presentation! Do you have any thoughts or predictions on which proteins RDN2 will interact with based on other research or the results you found during your experiments?
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