For Rfc1 to be affiliated with the DNA Damage Repair pathway, the gene would have to be upregulated or downregulated following DNA Damage, demonstrated by either a higher or lower band intensity of the LMN HU +RT sample compared to LMN UT +RT in Figure 2. An example of a gene upregulated following DNA Damage is our positive control (Rad51.)
Rfc1 could be a housekeeping gene that carries out basic cellular maintenance processes, requiring continuous expression even in the event of DNA Damage. However, additional research would need to be conducted to support this.
curesymposium.org 
If the DNA damage repair pathway had been affected by this gene, how would your results have differed?
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For Rfc1 to be affiliated with the DNA Damage Repair pathway, the gene would have to be upregulated or downregulated following DNA Damage, demonstrated by either a higher or lower band intensity of the LMN HU +RT sample compared to LMN UT +RT in Figure 2. An example of a gene upregulated following DNA Damage is our positive control (Rad51.)
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Do you believe the gene serves a different function within the various DNA pathways that would correlate to it expression in the repair pathway?
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Rfc1 could be a housekeeping gene that carries out basic cellular maintenance processes, requiring continuous expression even in the event of DNA Damage. However, additional research would need to be conducted to support this.
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