I thought your presentation was very interesting! I was wondering what other applications this type of experiment could be used for in a more general sense and how this research applies to more of the bigger picture in learning about cell biology?
when applying our research to the bigger picture i think of DNA damage repair. In being able to target specific proteins/ molecules involved in DNA damage repair, i think of genome targeting therapies. Increasing the amount of a specific gene sequence could increase the volume of repair done by a cell/ increase efficiency of repair.
I really enjoyed your presentation! What did you mean by non specific binding when describing your first figure? What effect did this have on your results?
Non-specific binding refers to our primers bring to a region other than our desired region (target genome of twi8). Due to our research focusing on cDNA and not gDNA the non-specific binding did not have an impact on our results. Most likely an exon contained a similar sequence and some of the primer was able to anneal.
great question! I think if i could redo this experiment, i would chose to use the cDNA we isolated in lab instead of the lab provided cDNA. We wanted to ensure we got the expected results.
curesymposium.org 
I thought your presentation was very interesting! I was wondering what other applications this type of experiment could be used for in a more general sense and how this research applies to more of the bigger picture in learning about cell biology?
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when applying our research to the bigger picture i think of DNA damage repair. In being able to target specific proteins/ molecules involved in DNA damage repair, i think of genome targeting therapies. Increasing the amount of a specific gene sequence could increase the volume of repair done by a cell/ increase efficiency of repair.
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Great Presentation, What animals would you want to use DNA from when conducting further research?
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When BLAST was performed there were many similar sequences in drosophila and mice, so i would start there!
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I really enjoyed your presentation! What did you mean by non specific binding when describing your first figure? What effect did this have on your results?
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Non-specific binding refers to our primers bring to a region other than our desired region (target genome of twi8). Due to our research focusing on cDNA and not gDNA the non-specific binding did not have an impact on our results. Most likely an exon contained a similar sequence and some of the primer was able to anneal.
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Great Presentation! I was curious, if you could redo this experimentation and analysis, what would you chose to do differently?
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great question! I think if i could redo this experiment, i would chose to use the cDNA we isolated in lab instead of the lab provided cDNA. We wanted to ensure we got the expected results.
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