Many trials would have to be done before using the same sort of experiments on human cells, but we would most likely expect the results to be similar to the results gathered when experimenting on T. thermophila. This is because the gene Rrm2 in T. thermophila has a true human homolog (very veyr low E-value = similar DNA sequence) with the similar gene in humans.
Great question! Water in place of a DNA template allows us to see if there is contamination in any of our reagents such as primers, GoTaq, or PCR water.
Our gene, Rrm2, shows a similar expression pattern to Rad51 (as seen the figure 1) which is known to be involved in the DNA damage repair pathway. This led us to believe that Rrm2 may have similar function to Rad51.
curesymposium.org 
How do you think the results would change if you used this information to perform a similar experiment on humans?
LikeLike
Many trials would have to be done before using the same sort of experiments on human cells, but we would most likely expect the results to be similar to the results gathered when experimenting on T. thermophila. This is because the gene Rrm2 in T. thermophila has a true human homolog (very veyr low E-value = similar DNA sequence) with the similar gene in humans.
LikeLike
What is the benefit of using water as a negative control?
LikeLike
Great question! Water in place of a DNA template allows us to see if there is contamination in any of our reagents such as primers, GoTaq, or PCR water.
LikeLike
Great job, what relationship does the gene have to other genes related to DNA repair?
LikeLike
Our gene, Rrm2, shows a similar expression pattern to Rad51 (as seen the figure 1) which is known to be involved in the DNA damage repair pathway. This led us to believe that Rrm2 may have similar function to Rad51.
LikeLike
How would you use qPCR to determine homologs with other genes?
LikeLike