Super interesting topic! I remember studying this when I took Cell Bio lab in 2021.
I did have one question for you: What method or methods did you use to develop your primer sequence/sequences and how did you narrow it down to the one used in your experimental research?
We used software programs BLAST and Primer3Plus to design a primer pair and looked at things like product and primer size, GC%, etc. We ultimately chose this primer because it formed the least amount of secondary structures.
Great presentation! If your model organism undergoes DNA damage repair, how can you tell which portions of the DNA your developed primers and compounds fixed versus the damage that was pre-existing or occurring due to natural causes? Maybe I’m misunderstanding, but hopefully this question isn’t too complex!
We were looking at a specific gene of our model organism to see if it was involved in DNA damage or not. Our primers only bound to the specific gene sequence- they didn’t induce DNA damage. The hydroxyurea some samples were treated with is what caused the damage. We couldn’t tell what part of the DNA was damaged, but we compared the expression levels before DNA damage was induced and after to see if DNA repair maybe did happen. Hope that answered your question!
My comments aren’t posting so I’m sorry if I double comment! Great presentation! I was wondering if your model organism undergoes DNA damage repair already, how will you be able to tell what repair has been done by your compound versus what’s naturally happening to the model?
Hi Christina!
Since the Hypedc3 gene isn’t used in DNA damage repair pathways and so little is known about it, how would you conduct research to find the purpose of this gene and it’s pathway?
curesymposium.org 
What made you chose this gene in particular to study?
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we were assigned our genes!
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Hello Cheng!
Super interesting topic! I remember studying this when I took Cell Bio lab in 2021.
I did have one question for you: What method or methods did you use to develop your primer sequence/sequences and how did you narrow it down to the one used in your experimental research?
Look forward to seeing your response!
Cristian Joya
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We used software programs BLAST and Primer3Plus to design a primer pair and looked at things like product and primer size, GC%, etc. We ultimately chose this primer because it formed the least amount of secondary structures.
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Great presentation! If your model organism undergoes DNA damage repair, how can you tell which portions of the DNA your developed primers and compounds fixed versus the damage that was pre-existing or occurring due to natural causes? Maybe I’m misunderstanding, but hopefully this question isn’t too complex!
LikeLike
We were looking at a specific gene of our model organism to see if it was involved in DNA damage or not. Our primers only bound to the specific gene sequence- they didn’t induce DNA damage. The hydroxyurea some samples were treated with is what caused the damage. We couldn’t tell what part of the DNA was damaged, but we compared the expression levels before DNA damage was induced and after to see if DNA repair maybe did happen. Hope that answered your question!
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My comments aren’t posting so I’m sorry if I double comment! Great presentation! I was wondering if your model organism undergoes DNA damage repair already, how will you be able to tell what repair has been done by your compound versus what’s naturally happening to the model?
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Hi Christina!
Since the Hypedc3 gene isn’t used in DNA damage repair pathways and so little is known about it, how would you conduct research to find the purpose of this gene and it’s pathway?
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I’m not really sure, but maybe look at where in the cell the gene is expressed or compare its sequence to other genes with known functions.
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If you could would you want to have done a different gene or do you not believe that it would really change much for the labs intents and purposes?
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Finding a gene that was expressed more after DNA damage was induced would have been cool, but the methods used would have been the same for this lab.
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