Hi! The gene expression profile does. Conjugation is the exchange of genetic material, which requires lots of DNA damage and repair. Conjugation happens on a gene expression profile from C0-C18, and Rrm2 is highly expressed during those stages!
I believe it may be due to error in creating PCR samples. The other samples were all as expected meaning that the issue was not with running gel electrophoresis. It was very unfortunate, we were very excited for the results!
RPPO and Rad51 act as positive controls! Without both, or one, of the positive controls we cannot make definite conclusions based off of the gel. It may seem insignificant, but it means we cannot further our research until the error with controls is eliminated and the results with Rrm2 are still the same. Thank you for your time!
curesymposium.org 
What part of the profile indicates that Rrm2 would be involved with the repair pathway?
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Hi! The gene expression profile does. Conjugation is the exchange of genetic material, which requires lots of DNA damage and repair. Conjugation happens on a gene expression profile from C0-C18, and Rrm2 is highly expressed during those stages!
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Good Job!
What results would you expect if you run the experiments again? What’s to stop the error within RPPO and Rad51 primer samples?
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What do you think the Rad51 as the primer came out not as expected?
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I believe it may be due to error in creating PCR samples. The other samples were all as expected meaning that the issue was not with running gel electrophoresis. It was very unfortunate, we were very excited for the results!
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How would the presence of RPPO affect the experiment? Would this cause some small error, or is it more significant than that?
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RPPO and Rad51 act as positive controls! Without both, or one, of the positive controls we cannot make definite conclusions based off of the gel. It may seem insignificant, but it means we cannot further our research until the error with controls is eliminated and the results with Rrm2 are still the same. Thank you for your time!
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