We used Primer3Plus, Benchling, and BLAST to see what sequence would anneal to an exon in our genome and also ensure that the primers wouldn’t anneal to itself or eachother
That there was not enough expression under normal conditions but since the other controls ran as expected, we were able to determine that the PCR was performed correctly and it was just an expression issue
I am not too sure why our gene was not expressed in normal growing conditions, it could just be that our gene isn’t as necessary or primarily used in the cell for DNA damage repair. This is a normal finding and was not much cause for concern in our research since it was expressed after DNA damage was induced on the cells
What applications will this research on the TWI8 gene being part of the DNA response pathway have on the future? After further research, would this allow us to repair damaged DNA?
In the future it can be determined if TWI8 is directly and actively participating in DNA damage repair or if it is acting as a recruiter protein signaling other proteins to come repair the DNA
The function of TWI8 is important because it is unknown if our protein is a recruiter protein signaling other proteins to repair the DNA damage or if our protein is actively repairing the damage
The unexpected result could have been the conditions we set for our cells to grow in. Expression was increased after DNA damage because these genes are known to be involved in the DNA repair pathway for T. thermophila, so more expression means more involvement or presence. Our gene was suggested to be included due to the increase of expression
curesymposium.org 
How did you design the primers used?
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We used Primer3Plus, Benchling, and BLAST to see what sequence would anneal to an exon in our genome and also ensure that the primers wouldn’t anneal to itself or eachother
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What did the control verify about your RNA?
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That there was not enough expression under normal conditions but since the other controls ran as expected, we were able to determine that the PCR was performed correctly and it was just an expression issue
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Why was your gene not expressed in normal growing conditions? Is this normal?
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I am not too sure why our gene was not expressed in normal growing conditions, it could just be that our gene isn’t as necessary or primarily used in the cell for DNA damage repair. This is a normal finding and was not much cause for concern in our research since it was expressed after DNA damage was induced on the cells
LikeLike
What applications will this research on the TWI8 gene being part of the DNA response pathway have on the future? After further research, would this allow us to repair damaged DNA?
LikeLike
In the future it can be determined if TWI8 is directly and actively participating in DNA damage repair or if it is acting as a recruiter protein signaling other proteins to come repair the DNA
LikeLike
Why would knowing where Twi8 specifically functions be important?
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The function of TWI8 is important because it is unknown if our protein is a recruiter protein signaling other proteins to repair the DNA damage or if our protein is actively repairing the damage
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How do you think the unexpected results arose? Why is the expression changed so much in one of the genes?
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The unexpected result could have been the conditions we set for our cells to grow in. Expression was increased after DNA damage because these genes are known to be involved in the DNA repair pathway for T. thermophila, so more expression means more involvement or presence. Our gene was suggested to be included due to the increase of expression
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