Hi Clare,
Yes, I expected to see a band at 255 bp and a brighter band at the same size after DNA damage induced with Hydroxyurea. This means that there was definitely an expression after DNA damage.
Hi Katelyn,
We quantified expression using Fiji by adjusting brightness and contrast and measuring intensity. After that, we obtained numbers that we analyzed according to a table in Excel that our instructor provided, removing the background and normalizing the information obtained.
Hi Logan,
I think that it is necessary for DNA damage since Rdn2 plays an important role in converting single-stranded DNA to double-stranded DNA and then small RNAs, which help in the DNA repair pathway after damage.
Hi Morgan,
In the second column, we have our Gene-specific (Rdn2) untreated, in this case, we obtained an expression ( a band present at 255 bp). In the third column, we have our Gene-specific (Rdn2) and Hydroxyurea (DNA reagent damage), this band at 255 bp is brighter, meaning that there was an increase in expression after DNA damage.
curesymposium.org 
did you expect there to be a band at 255bp in GS UT RT+
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Hi Clare,
Yes, I expected to see a band at 255 bp and a brighter band at the same size after DNA damage induced with Hydroxyurea. This means that there was definitely an expression after DNA damage.
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How do you measure the amount of DNA expression, or is it all relative?
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Hi Katelyn,
We quantified expression using Fiji by adjusting brightness and contrast and measuring intensity. After that, we obtained numbers that we analyzed according to a table in Excel that our instructor provided, removing the background and normalizing the information obtained.
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Do you think that Rdn2 is needed for general DNA damage, or is it specific to the cuts made in this experiment?
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Hi Logan,
I think that it is necessary for DNA damage since Rdn2 plays an important role in converting single-stranded DNA to double-stranded DNA and then small RNAs, which help in the DNA repair pathway after damage.
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How did you determine that having a band in two different lanes at 255bp showed an increase in gene expression?
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Hi Morgan,
In the second column, we have our Gene-specific (Rdn2) untreated, in this case, we obtained an expression ( a band present at 255 bp). In the third column, we have our Gene-specific (Rdn2) and Hydroxyurea (DNA reagent damage), this band at 255 bp is brighter, meaning that there was an increase in expression after DNA damage.
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