The DNA damage was induced by adding 2ul of the chemical hydroxyurea (HU) to a sample of 1ml of T. thermophila for 1hr. We then stopped the DNA damaging process by putting the cells through the lysing process (the breaking apart of the cells) in order to extract the RNA before we converted it to cDNA vis RT-PCR.
In your video, you mentioned that your research concluded that TWI7 is more likely involved in environments outside of normal conditions. What determines a “normal condition” vs. a non-normal condition?
T. thermophila can live in a wide variety of environments. A non-normal condition would be thermal hot springs (high temperatures), soil, and even sewage (extreme pHs and toxic chemicals). The normal conditions for T. thermophila very more widely than other ciliate species but this would would range from ponds, freshwater, and brackish water.
Really nice poster! how would your data differ if you had choose a different model organism? like for example if it had slower generation time as opposed to the fast generation time your organism had
Thank you! For our specific gene I do not believe our gene is found exactly in any other organisms, although there are genes that are similar. If we studied another gene from a different model organism there would have been a more difficult process in lab containment and even extraction of the DNA. Especially if it was a larger model organism I believe the time if would take would have exceeded our semester limitations, or by a minimum required more lab equipment not available to our specific lab.
curesymposium.org 
How was the DNA damage induced before you converted it to cDNA?
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The DNA damage was induced by adding 2ul of the chemical hydroxyurea (HU) to a sample of 1ml of T. thermophila for 1hr. We then stopped the DNA damaging process by putting the cells through the lysing process (the breaking apart of the cells) in order to extract the RNA before we converted it to cDNA vis RT-PCR.
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Great job following through the poster smoothly with confidence! How did you induce the DNA damage in the cells?
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Thanks you! So we used Hydroxyurea (HU) which is a well used chemical agent to damage DNA.
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In your video, you mentioned that your research concluded that TWI7 is more likely involved in environments outside of normal conditions. What determines a “normal condition” vs. a non-normal condition?
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T. thermophila can live in a wide variety of environments. A non-normal condition would be thermal hot springs (high temperatures), soil, and even sewage (extreme pHs and toxic chemicals). The normal conditions for T. thermophila very more widely than other ciliate species but this would would range from ponds, freshwater, and brackish water.
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Really nice poster! how would your data differ if you had choose a different model organism? like for example if it had slower generation time as opposed to the fast generation time your organism had
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Thank you! For our specific gene I do not believe our gene is found exactly in any other organisms, although there are genes that are similar. If we studied another gene from a different model organism there would have been a more difficult process in lab containment and even extraction of the DNA. Especially if it was a larger model organism I believe the time if would take would have exceeded our semester limitations, or by a minimum required more lab equipment not available to our specific lab.
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Great presentation!
How would you determine what down stream targets and pathways are regulated by TWI7 as mentioned in the future directions?
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