Hypedc3 was previously uncharacterized, so the purpose of our research was to determine whether or not it is involved in the DNA damage response. The results of our experiments determined that it isn’t involved in these pathways
A primer validation is performed through a PCR experiment, where gene-selected primers are added to a sample of DNA template. If amplification occurs at the expected product size, the primers are validated for the purpose of amplifying its specific gene.
Expected results were that the gene-specific primers would amplify the Hypedc3 gene at approximately 172 base pairs, and assuming that Hypedc3 is involved in the DNA damage response, that expression of Hypedc3 would increase after DNA damage is induced.
curesymposium.org 
Why did you chose Hypedc3 if it has no role in DNA damage repair?
LikeLike
Hypedc3 was previously uncharacterized, so the purpose of our research was to determine whether or not it is involved in the DNA damage response. The results of our experiments determined that it isn’t involved in these pathways
LikeLike
How was the Hypedc3 gene chosen? Is everybody in cell bio assigned a gene from the same organism?
LikeLike
Each lab group was assigned a different, previously uncharacterized gene from the Tetrahymena thermophila organism
LikeLike
How is a primer validation performed?
LikeLike
A primer validation is performed through a PCR experiment, where gene-selected primers are added to a sample of DNA template. If amplification occurs at the expected product size, the primers are validated for the purpose of amplifying its specific gene.
LikeLike
What were your expected results, prior to beginning your experiment?
LikeLike
Expected results were that the gene-specific primers would amplify the Hypedc3 gene at approximately 172 base pairs, and assuming that Hypedc3 is involved in the DNA damage response, that expression of Hypedc3 would increase after DNA damage is induced.
LikeLike