Great question! qPCR is another form of PCR that essentially utilizes an additional reagent and a fancier thermocycler that can put the PCR samples through many more cycles. Due to all the additional cycles, lowly expressed genes might show up as light bands that were not visible before because not enough PCR cycles had occurred to amplify it.
Hello! I really enjoyed listening to your presentation, amazing job! A question that I have is in regards to the use of the T. thermophilia cells. If you were to conduct this experiment again, do you have another cell in mind that you would like to try to see how it affects the expression of the TWI7 gene? Thank you!
Good question! It would have to be a cell that the TWI7 gene was present in, so pulling up a homology chart would be helpful in answering your question. Maybe TWI7 would play different roles depending on the species of cell it was taken from, and if I had to guess it might be within a mouse cell since we had some homology come up between T. thermophila and mice/rats.
Thank you! Ribosomal RNA miniprep was the name of the procedure that our lab followed in order to extract RNA from our T. thermophila cells- it is just the manufacturer protocol.
Thank you! RT-PCR is the PCR technique we used in the lab that is described in the procedure section; we wanted to repeat it since our positive control was not valid for our final PCR. An additional type of PCR aside from the one I briefly discussed performing in the lab (with a thermocycler that doesn’t cycle as much as others and specific reagents) is qPCR. qPCR is another form of PCR that essentially utilizes an additional reagent apart from those used by RT-PCR and a fancier thermocycler that can put the PCR samples through many more cycles. Due to all the additional cycles, lowly expressed genes might show up as light bands that were not visible before because not enough PCR cycles had occurred to amplify it.
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Hi! Your poster and presentation are really good! The only question I have is what is qPCR and how would it help you come up with results?
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Great question! qPCR is another form of PCR that essentially utilizes an additional reagent and a fancier thermocycler that can put the PCR samples through many more cycles. Due to all the additional cycles, lowly expressed genes might show up as light bands that were not visible before because not enough PCR cycles had occurred to amplify it.
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Hello! I really enjoyed listening to your presentation, amazing job! A question that I have is in regards to the use of the T. thermophilia cells. If you were to conduct this experiment again, do you have another cell in mind that you would like to try to see how it affects the expression of the TWI7 gene? Thank you!
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Good question! It would have to be a cell that the TWI7 gene was present in, so pulling up a homology chart would be helpful in answering your question. Maybe TWI7 would play different roles depending on the species of cell it was taken from, and if I had to guess it might be within a mouse cell since we had some homology come up between T. thermophila and mice/rats.
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I love the organization and flow of your presentation. What is ribosomal RNA prep that you discussed around 3:35? Thank you!
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Thank you! Ribosomal RNA miniprep was the name of the procedure that our lab followed in order to extract RNA from our T. thermophila cells- it is just the manufacturer protocol.
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This was a super good and informative presentation! I would like to know more about what RT-PR is?
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Thank you! RT-PCR is the PCR technique we used in the lab that is described in the procedure section; we wanted to repeat it since our positive control was not valid for our final PCR. An additional type of PCR aside from the one I briefly discussed performing in the lab (with a thermocycler that doesn’t cycle as much as others and specific reagents) is qPCR. qPCR is another form of PCR that essentially utilizes an additional reagent apart from those used by RT-PCR and a fancier thermocycler that can put the PCR samples through many more cycles. Due to all the additional cycles, lowly expressed genes might show up as light bands that were not visible before because not enough PCR cycles had occurred to amplify it.
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