that is a great question! and to be honest we, and the TA’s, were not sure because it did not show up in the other -RT samples for the RPP0 and RAD 51 which is what would have happened if it was gDNA contamination. It was either a loading issue in the gal or some miss pipetting for that specific tube.
Two questions:
1. What was the cause of contamination on your first two trials and how did you eliminate it?
2. How was protein assay further this research?
the first issue was caused by a miss loading of the gel we believe. the second contamination was quite confusing actually and we think it was the wrong DNA sample was loading into the tube but we are unsure to be honest.
a protein assay would show us gene expression which can be more direct and influential then mRNA expression
good question, we are definitely not sure it is involved in DNA repair. the fact that it is up regulated when damage happens is a good correlation but that is it.
curesymposium.org 
What do you hypothesize caused the contamination in the -RT samples of the first two gels?
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that is a great question! and to be honest we, and the TA’s, were not sure because it did not show up in the other -RT samples for the RPP0 and RAD 51 which is what would have happened if it was gDNA contamination. It was either a loading issue in the gal or some miss pipetting for that specific tube.
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How did you choose RSP1 as your gene? Was it harder to analyze because not a lot was known about it?
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we were assigned this gene. and yes, it was a little bit harder because of the lack of analogs and information but that was exciting in it’s own way!
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Two questions:
1. What was the cause of contamination on your first two trials and how did you eliminate it?
2. How was protein assay further this research?
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the first issue was caused by a miss loading of the gel we believe. the second contamination was quite confusing actually and we think it was the wrong DNA sample was loading into the tube but we are unsure to be honest.
a protein assay would show us gene expression which can be more direct and influential then mRNA expression
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Would the contamination found cause further problems for future experiments?
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Good question. The contamination made the results inviable which meant we could not move forward. thanks!
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How did you know that Rsp1 was responsible for DNA repair?
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good question, we are definitely not sure it is involved in DNA repair. the fact that it is up regulated when damage happens is a good correlation but that is it.
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